Mechanisms of action of two different natural mixtures of persistent organic pollutants (POPs) in ovarian follicles.
Identifieur interne : 000519 ( Main/Exploration ); précédent : 000518; suivant : 000520Mechanisms of action of two different natural mixtures of persistent organic pollutants (POPs) in ovarian follicles.
Auteurs : E L Gregoraszczuk [Pologne] ; A. Ptak ; J U Skaare ; K. Mularz ; A. Chmielowiec ; A. Wojtowicz ; E. RopstadSource :
- Xenobiotica; the fate of foreign compounds in biological systems [ 1366-5928 ] ; 2009.
Descripteurs français
- KwdFr :
- Activation enzymatique (MeSH), Animaux (MeSH), Cellules cultivées (MeSH), Cytochrome P-450 enzyme system (composition chimique), Cytochrome P-450 enzyme system (métabolisme), Extrait cellulaire (toxicité), Femelle (MeSH), Follicule ovarique (effets des médicaments et des substances chimiques), Follicule ovarique (métabolisme), Gonadotrophines (pharmacologie), Mélanges complexes (composition chimique), Mélanges complexes (toxicité), Oestradiol (métabolisme), Polluants environnementaux (toxicité), Polychlorobiphényles (analyse), Polychlorobiphényles (toxicité), Récepteur bêta des oestrogènes (agonistes), Récepteur bêta des oestrogènes (analyse), Récepteurs à hydrocarbure aromatique (agonistes), Récepteurs à hydrocarbure aromatique (composition chimique), Suidae (MeSH), Survie cellulaire (physiologie), Éthers de polyhalogénophényle (analyse), Éthers de polyhalogénophényle (toxicité).
- MESH :
- agonistes : Récepteur bêta des oestrogènes, Récepteurs à hydrocarbure aromatique.
- analyse : Polychlorobiphényles, Récepteur bêta des oestrogènes, Éthers de polyhalogénophényle.
- composition chimique : Cytochrome P-450 enzyme system, Mélanges complexes, Récepteurs à hydrocarbure aromatique.
- effets des médicaments et des substances chimiques : Follicule ovarique.
- métabolisme : Cytochrome P-450 enzyme system, Follicule ovarique, Oestradiol.
- pharmacologie : Gonadotrophines.
- physiologie : Survie cellulaire.
- toxicité : Extrait cellulaire, Mélanges complexes, Polluants environnementaux, Polychlorobiphényles, Éthers de polyhalogénophényle.
- Activation enzymatique, Animaux, Cellules cultivées, Femelle, Suidae.
English descriptors
- KwdEn :
- Animals (MeSH), Cell Extracts (toxicity), Cell Survival (physiology), Cells, Cultured (MeSH), Complex Mixtures (chemistry), Complex Mixtures (toxicity), Cytochrome P-450 Enzyme System (chemistry), Cytochrome P-450 Enzyme System (metabolism), Environmental Pollutants (toxicity), Enzyme Activation (MeSH), Estradiol (metabolism), Estrogen Receptor beta (agonists), Estrogen Receptor beta (analysis), Female (MeSH), Gonadotropins (pharmacology), Halogenated Diphenyl Ethers (analysis), Halogenated Diphenyl Ethers (toxicity), Ovarian Follicle (drug effects), Ovarian Follicle (metabolism), Polychlorinated Biphenyls (analysis), Polychlorinated Biphenyls (toxicity), Receptors, Aryl Hydrocarbon (agonists), Receptors, Aryl Hydrocarbon (chemistry), Swine (MeSH).
- MESH :
- chemical , agonists : Estrogen Receptor beta, Receptors, Aryl Hydrocarbon.
- chemical , analysis : Estrogen Receptor beta, Halogenated Diphenyl Ethers, Polychlorinated Biphenyls.
- chemical , chemistry : Complex Mixtures, Cytochrome P-450 Enzyme System, Receptors, Aryl Hydrocarbon.
- chemical , metabolism : Cytochrome P-450 Enzyme System, Estradiol.
- chemical , pharmacology : Gonadotropins.
- chemical , toxicity : Cell Extracts, Complex Mixtures, Environmental Pollutants, Halogenated Diphenyl Ethers, Polychlorinated Biphenyls.
- drug effects : Ovarian Follicle.
- metabolism : Ovarian Follicle.
- physiology : Cell Survival.
- Animals, Cells, Cultured, Enzyme Activation, Female, Swine.
Abstract
The present work investigated the effects of two different natural mixtures on aryl hydrocarbon receptor (AhR) and oestrogen receptor (ER)beta protein levels, as well as on the activity of cytochrome P450 (CYP) 1A1 and CYP2B. Consequently, the authors observed the effects of these mixtures on gonadotropine-stimulated steroid secretion by ovarian follicles. The natural mixtures that were studied were 'Mjosa' extracted from burbot liver, which contains a high level of PBDEs, and 'Marine mix', extracted from Atlantic cod liver, which contains a high level of polychlorinated biphenyls (PCBs). Follicular cells were exposed in vitro to 'Marine mix' and 'Mjosa mix' at doses of 3.6 and 1.4 microg ml(-1), respectively. Media were collected and used for steroid analysis and cell viability assays. Cells were used to estimate aromatase activity (CYP19), AhR and ER protein levels, and CYP1A1 and CYP2B1 activity. Western blot analysis indicated down-regulation of AhR by 'Marine mix' and down-regulation of ERbeta by Mjosa mix. Up-regulation of CYP1A1 expression and activity were seen following treatment with Marine mix, but not Mjosa mix. Increased CYP2B1 activity was noted after treatment with both 'Marine mix' and Mjosa mix. Both mixtures increased luteinizing hormone (LH)-stimulated progesterone and testosterone secretion, follicle-stimulating hormone (FSH)-stimulated oestradiol secretion, and CYP19 activity. These results suggest that: (1) 'Marine mix' is a mixed-type CYP inducer; (2) 'Mjosa mix' is an inducer of ERbeta and CYP2B; and (3) both 'Marine mix' and 'Mjosa mix' stimulate aromatase activity as a consequence of oestradiol secretion through activation of CYP19.
DOI: 10.1080/00498250802578468
PubMed: 19219750
Affiliations:
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Ptak</name></author><author><name sortKey="Skaare, J U" sort="Skaare, J U" uniqKey="Skaare J" first="J U" last="Skaare">J U Skaare</name></author><author><name sortKey="Mularz, K" sort="Mularz, K" uniqKey="Mularz K" first="K" last="Mularz">K. Mularz</name></author><author><name sortKey="Chmielowiec, A" sort="Chmielowiec, A" uniqKey="Chmielowiec A" first="A" last="Chmielowiec">A. Chmielowiec</name></author><author><name sortKey="Wojtowicz, A" sort="Wojtowicz, A" uniqKey="Wojtowicz A" first="A" last="Wojtowicz">A. Wojtowicz</name></author><author><name sortKey="Ropstad, E" sort="Ropstad, E" uniqKey="Ropstad E" first="E" last="Ropstad">E. Ropstad</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="doi">10.1080/00498250802578468</idno><idno type="RBID">pubmed:19219750</idno><idno type="pmid">19219750</idno><idno type="wicri:Area/PubMed/Corpus">000078</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000078</idno><idno type="wicri:Area/PubMed/Curation">000078</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000078</idno><idno type="wicri:Area/PubMed/Checkpoint">000081</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000081</idno><idno type="wicri:Area/Ncbi/Merge">000141</idno><idno type="wicri:Area/Ncbi/Curation">000141</idno><idno type="wicri:Area/Ncbi/Checkpoint">000141</idno><idno type="wicri:Area/Main/Merge">000525</idno><idno type="wicri:Area/Main/Curation">000519</idno><idno type="wicri:Area/Main/Exploration">000519</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mechanisms of action of two different natural mixtures of persistent organic pollutants (POPs) in ovarian follicles.</title><author><name sortKey="Gregoraszczuk, E L" sort="Gregoraszczuk, E L" uniqKey="Gregoraszczuk E" first="E L" last="Gregoraszczuk">E L Gregoraszczuk</name><affiliation wicri:level="1"><nlm:affiliation>Departament of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Kraków, Poland. ewa.gregoraszczuk@uj.edu.pl</nlm:affiliation><country xml:lang="fr">Pologne</country><wicri:regionArea>Departament of Physiology and Toxicology of Reproduction, Institute of Zoology, Jagiellonian University, Kraków</wicri:regionArea><wicri:noRegion>Kraków</wicri:noRegion></affiliation></author><author><name sortKey="Ptak, A" sort="Ptak, A" uniqKey="Ptak A" first="A" last="Ptak">A. Ptak</name></author><author><name sortKey="Skaare, J U" sort="Skaare, J U" uniqKey="Skaare J" first="J U" last="Skaare">J U Skaare</name></author><author><name sortKey="Mularz, K" sort="Mularz, K" uniqKey="Mularz K" first="K" last="Mularz">K. Mularz</name></author><author><name sortKey="Chmielowiec, A" sort="Chmielowiec, A" uniqKey="Chmielowiec A" first="A" last="Chmielowiec">A. Chmielowiec</name></author><author><name sortKey="Wojtowicz, A" sort="Wojtowicz, A" uniqKey="Wojtowicz A" first="A" last="Wojtowicz">A. Wojtowicz</name></author><author><name sortKey="Ropstad, E" sort="Ropstad, E" uniqKey="Ropstad E" first="E" last="Ropstad">E. Ropstad</name></author></analytic><series><title level="j">Xenobiotica; the fate of foreign compounds in biological systems</title><idno type="eISSN">1366-5928</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Cell Extracts (toxicity)</term><term>Cell Survival (physiology)</term><term>Cells, Cultured (MeSH)</term><term>Complex Mixtures (chemistry)</term><term>Complex Mixtures (toxicity)</term><term>Cytochrome P-450 Enzyme System (chemistry)</term><term>Cytochrome P-450 Enzyme System (metabolism)</term><term>Environmental Pollutants (toxicity)</term><term>Enzyme Activation (MeSH)</term><term>Estradiol (metabolism)</term><term>Estrogen Receptor beta (agonists)</term><term>Estrogen Receptor beta (analysis)</term><term>Female (MeSH)</term><term>Gonadotropins (pharmacology)</term><term>Halogenated Diphenyl Ethers (analysis)</term><term>Halogenated Diphenyl Ethers (toxicity)</term><term>Ovarian Follicle (drug effects)</term><term>Ovarian Follicle (metabolism)</term><term>Polychlorinated Biphenyls (analysis)</term><term>Polychlorinated Biphenyls (toxicity)</term><term>Receptors, Aryl Hydrocarbon (agonists)</term><term>Receptors, Aryl Hydrocarbon (chemistry)</term><term>Swine (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation enzymatique (MeSH)</term><term>Animaux (MeSH)</term><term>Cellules cultivées (MeSH)</term><term>Cytochrome P-450 enzyme system (composition chimique)</term><term>Cytochrome P-450 enzyme system (métabolisme)</term><term>Extrait cellulaire (toxicité)</term><term>Femelle (MeSH)</term><term>Follicule ovarique (effets des médicaments et des substances chimiques)</term><term>Follicule ovarique (métabolisme)</term><term>Gonadotrophines (pharmacologie)</term><term>Mélanges complexes (composition chimique)</term><term>Mélanges complexes (toxicité)</term><term>Oestradiol (métabolisme)</term><term>Polluants environnementaux (toxicité)</term><term>Polychlorobiphényles (analyse)</term><term>Polychlorobiphényles (toxicité)</term><term>Récepteur bêta des oestrogènes (agonistes)</term><term>Récepteur bêta des oestrogènes (analyse)</term><term>Récepteurs à hydrocarbure aromatique (agonistes)</term><term>Récepteurs à hydrocarbure aromatique (composition chimique)</term><term>Suidae (MeSH)</term><term>Survie cellulaire (physiologie)</term><term>Éthers de polyhalogénophényle (analyse)</term><term>Éthers de polyhalogénophényle (toxicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="agonists" xml:lang="en"><term>Estrogen Receptor beta</term><term>Receptors, Aryl Hydrocarbon</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Estrogen Receptor beta</term><term>Halogenated Diphenyl Ethers</term><term>Polychlorinated Biphenyls</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Complex Mixtures</term><term>Cytochrome P-450 Enzyme System</term><term>Receptors, Aryl Hydrocarbon</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cytochrome P-450 Enzyme System</term><term>Estradiol</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Gonadotropins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Cell Extracts</term><term>Complex Mixtures</term><term>Environmental Pollutants</term><term>Halogenated Diphenyl Ethers</term><term>Polychlorinated Biphenyls</term></keywords><keywords scheme="MESH" qualifier="agonistes" xml:lang="fr"><term>Récepteur bêta des oestrogènes</term><term>Récepteurs à hydrocarbure aromatique</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Polychlorobiphényles</term><term>Récepteur bêta des oestrogènes</term><term>Éthers de polyhalogénophényle</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Cytochrome P-450 enzyme system</term><term>Mélanges complexes</term><term>Récepteurs à hydrocarbure aromatique</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Ovarian Follicle</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Follicule ovarique</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Ovarian Follicle</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cytochrome P-450 enzyme system</term><term>Follicule ovarique</term><term>Oestradiol</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Gonadotrophines</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Survie cellulaire</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Cell Survival</term></keywords><keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Extrait cellulaire</term><term>Mélanges complexes</term><term>Polluants environnementaux</term><term>Polychlorobiphényles</term><term>Éthers de polyhalogénophényle</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cells, Cultured</term><term>Enzyme Activation</term><term>Female</term><term>Swine</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Activation enzymatique</term><term>Animaux</term><term>Cellules cultivées</term><term>Femelle</term><term>Suidae</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The present work investigated the effects of two different natural mixtures on aryl hydrocarbon receptor (AhR) and oestrogen receptor (ER)beta protein levels, as well as on the activity of cytochrome P450 (CYP) 1A1 and CYP2B. Consequently, the authors observed the effects of these mixtures on gonadotropine-stimulated steroid secretion by ovarian follicles. The natural mixtures that were studied were 'Mjosa' extracted from burbot liver, which contains a high level of PBDEs, and 'Marine mix', extracted from Atlantic cod liver, which contains a high level of polychlorinated biphenyls (PCBs). Follicular cells were exposed in vitro to 'Marine mix' and 'Mjosa mix' at doses of 3.6 and 1.4 microg ml(-1), respectively. Media were collected and used for steroid analysis and cell viability assays. Cells were used to estimate aromatase activity (CYP19), AhR and ER protein levels, and CYP1A1 and CYP2B1 activity. Western blot analysis indicated down-regulation of AhR by 'Marine mix' and down-regulation of ERbeta by Mjosa mix. Up-regulation of CYP1A1 expression and activity were seen following treatment with Marine mix, but not Mjosa mix. Increased CYP2B1 activity was noted after treatment with both 'Marine mix' and Mjosa mix. Both mixtures increased luteinizing hormone (LH)-stimulated progesterone and testosterone secretion, follicle-stimulating hormone (FSH)-stimulated oestradiol secretion, and CYP19 activity. These results suggest that: (1) 'Marine mix' is a mixed-type CYP inducer; (2) 'Mjosa mix' is an inducer of ERbeta and CYP2B; and (3) both 'Marine mix' and 'Mjosa mix' stimulate aromatase activity as a consequence of oestradiol secretion through activation of CYP19.</div></front></TEI><affiliations><list><country><li>Pologne</li></country></list><tree><noCountry><name sortKey="Chmielowiec, A" sort="Chmielowiec, A" uniqKey="Chmielowiec A" first="A" last="Chmielowiec">A. Chmielowiec</name><name sortKey="Mularz, K" sort="Mularz, K" uniqKey="Mularz K" first="K" last="Mularz">K. Mularz</name><name sortKey="Ptak, A" sort="Ptak, A" uniqKey="Ptak A" first="A" last="Ptak">A. Ptak</name><name sortKey="Ropstad, E" sort="Ropstad, E" uniqKey="Ropstad E" first="E" last="Ropstad">E. Ropstad</name><name sortKey="Skaare, J U" sort="Skaare, J U" uniqKey="Skaare J" first="J U" last="Skaare">J U Skaare</name><name sortKey="Wojtowicz, A" sort="Wojtowicz, A" uniqKey="Wojtowicz A" first="A" last="Wojtowicz">A. Wojtowicz</name></noCountry><country name="Pologne"><noRegion><name sortKey="Gregoraszczuk, E L" sort="Gregoraszczuk, E L" uniqKey="Gregoraszczuk E" first="E L" last="Gregoraszczuk">E L Gregoraszczuk</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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